Centrifuge Tube Factory Introduces The Use Process Of Plastic Reagent Bottles
2023-1-7
Comparison Of Two Raw Materials Commonly Used In Plastic Reagent Bottle With the continuous expansion of the application scope of various polymer materials, Plastic Reagent Bottle has gradually been widely used in the storage of chemical reagents. polypropylene (PP), and high-density polyethylene (HDPE) are two commonly used materials. Centrifuge Tube Factory introduces What is the difference in performance between these two materials?
1. Withstand the temperature
The embrittlement temperature of
HDPE is -100 °C, and the embrittlement temperature of PP is 0 °C. Therefore,
when the product needs to be stored at low temperatures, more reagent bottles
made of HDPE material are selected, such as the 2-8 °C buffer used for storage
in diagnostic reagents. Buffer and -20 ℃ enzyme reagent bottles; for products
that need to be used at high temperatures, choose PP material.
2. Chemical resistance
Reagent bottles made of HDPE and PP
are both resistant to acid and alkali at room temperature, but HDPE is superior
to PP in terms of oxidation resistance. Therefore, when storing oxidative
materials, use HDPE bottles; low molecular weight aliphatic hydrocarbons,
Aromatic hydrocarbons, and chlorinated hydrocarbons can soften and swell
polypropylene, so when storing organic solvents such as benzene rings, n-hexane,
and chlorinated hydrocarbons, HDPE plastic reagent bottles should be
used.
3. Toughness and impact resistance
Polypropylene (PP) has
excellent flexural fatigue resistance but poor impact resistance at low
temperatures. However, the drop resistance of the HDPE material reagent bottle
is much better than that of the PP material reagent bottle, so it is not
suitable to use the PP material bottle for low-temperature storage.
4. Transparency
Compared with HDPE, PP is more transparent,
which is more conducive to observing the state of the materials stored in the
bottle. However, the most transparent PP bottles currently on the market are all
made with a transparent agent, so you need to pay attention when choosing PP
material reagent bottles.
5. Sterilization method
The only difference between HDPE and
PP in the sterilization method is that PP can be sterilized by high temperature
and high pressure, while HDPE cannot. Both can be sterilized by EO, radiation
sterilization (irradiation-resistant PP is required, otherwise it will turn
yellow), and disinfection. agent sterilization. Whether it is a reagent bottle
made of PP or a reagent bottle made of HDPE, there are suitable types of
chemical reagents according to the characteristics of the material. When
selecting reagent bottles, the physical properties of chemical reagents should
be considered.
Through the above introduction, Urine Collector Manufacturers hopes that you can simply refer to the content of this article in future use.
---------------------
Pipette Tips Manufacturers Introduces Tips For Using Cryovials
2022-12-16
Freezing Tube is also known as a strain storage tube, magnetic bead storage tube, and magnetic bead cryopreservation tube. The commonly used strain preservation methods in the microbiology laboratory include the milk method, the glycerol method, and the slant method. The complexity varies greatly, and the effect varies greatly. At present, most laboratories in China make their own strain preservation tubes, which not only increases the work intensity but also due to various conditions, the strain preservation effect is not always satisfactory. Therefore, it is necessary to master the use of cryopreservation tubes and some precautions, so as to play a great role. Pipette Tips Manufacturers Introduces Precautions for the use of Freezing Tube:
1. The storage environment of cryopreservation tubes. Unused cryopreservation tubes can be stored at room temperature or 2-8°C for 12 months; inoculated cryotubes can be stored at -20°C, and good strains can be stored within 12 months. Effect: The inoculated cryopreservation tube can be stored at -80°C, and the strain can be preserved within 24 months.
2. Storage time of cryovials, unused cryovials can be stored at room temperature or 2-8°C; inoculated cryovials can be stored at -20°C or -80°C.
3. The steps of using the cryopreservation tube, pick the fresh culture from the pure bacterial culture and make it into a bacterial suspension with a McFarland turbidity of about 3-4 and inoculate it into the bacterial strain storage tube; tighten the storage tube and turn it upside down. 4-5 times to emulsify the bacteria, do not shake; put the preservation tube in the refrigerator (-20℃--70℃).
Through
the above introduction,Urine
Collector Factory hopes
that you can simply refer to the content of this article in future
use.
-------------------------------------
Plastic Reagent Bottle Manufacturers Introduces How To Use Pipettes
2022-11-3
There is no doubt that pipettes are one of the most important tools in biological research. They come in a variety of designs and are used in countless fields of research. While many liquid handling guides emphasize the importance of pipette maintenance, the pipetting technique is just as important. Below, Plastic Reagent Bottle Manufacturers explains how to use pipettes:
1. Choose the right pipette and tip: The match between the pipette and the pipette tip is the key to pipetting accuracy. If the tip and pipette do not match or use poor-quality tips, the seal between the tip and the pipette may be compromised. A good pipette tip ensures a proper seal to minimize sample loss due to leaks.
2. Properly calibrate the pipette according to the liquid density: If the density of the liquid differs greatly from that of the water, please recalibrate the pipette. If you are pipetting viscous liquids, be sure to pipette at a slower speed and work in reverse pipette mode. When pipetting volatile liquids, you need to pipette quickly and use the reverse pipette mode.
3. Pre-wet the tip: To reduce the loss of liquid hanging on the wall, pre-wet the tip at least 3 times. This method of infiltration helps to establish temperature balance and bring humidity into the dry space within the tip. Skipping this step may affect the accuracy of the dispensed volume due to evaporation or liquid retention.
4. Proper aspiration: Immerse the tip below the surface of the liquid. When sucking liquid, the tip should not be immersed too deep in the liquid, and the residence time in the liquid should be about 1S, because immersion too shallow or too deep may lead to inaccurate liquid suction.
5. Pause after each puff: A slow, even, sudden release helps reduce errors. Especially important when dealing with viscous liquids. As much as possible, try to pause for one to two seconds after each aspiration to allow the liquid level to stabilize in the tip.
6. In the use of the pipette, it is also necessary to pay attention to the inclination angle of the pipette: at the same time, pay attention to the inclination angle of the pipette during operation, try to ensure vertical suction, and keep the inclination angle range not greater than 20°. When aspirating liquid, try not to touch the wall of the container with the tip, especially when handling microscopic samples.
7. Adjust to appropriate gear: Use consistent and stable speed and pressure when handling pipettes and samples. In the design of Rainin pipettes, in order to ensure the accuracy of pipetting, two gears, the first gear, and the second gear, are set. You can choose the appropriate gear for pipetting operations according to your experiment and usage habits.
8. Pay attention to temperature: If your experiment allows, make sure that pipettes, tips, and liquids are all at room temperature. Changes in temperature and air pressure can also cause the air inside the pipette tip to contract or expand, which can affect the accuracy of dispensing liquids. Also, try to avoid overhandling pipettes and tips, as your body temperature may also affect the amount of liquid dispensed. After the pipetting operation is completed, it can be placed on the pipette holder, not on the laboratory bench.
9. Do a good job in daily maintenance and maintenance: Instrument calibration and daily maintenance are also effective means to prolong the life of the pipette, so it is recommended to clean and maintain the pipette on a weekly or monthly basis and calibrate regularly to improve its accuracy.
10. Develop good pipette operation habits: The development of pipetting operation habits is often ignored. Such habits include not only the correct and skilled use of pipette guns but also the posture of pipetting experiments. If the pipetting posture is Incorrect operation, repeated operations for a long time may cause your muscles to be in a tense state or even muscle strain. To solve this problem, you can choose an ergonomic pipette and develop good pipetting habits.
Through the above introduction,Collection Tube Manufacturers hopes that you can simply refer to the content of this article in future use.
----------------------------
Pipette Tips Manufacturers Introduce The Use Of Disposable Medical Consumables
2022-10-9
Pipette Tips Manufacturers introduce the management requirements for disposable medical consumables:
1. Control the total consumption of medical consumables and implement on-demand consumption. The amount received in the material warehouse of the pharmacy department shall not exceed the usage amount of 15 days at most, and the amount received in the supply room shall not exceed the usage amount of 7 days at most, so as to facilitate the accuracy of the hospital's accounting. Medical technology and clinical departments implement a pre-declaration system for materials that have been used in large quantities recently and explain the reasons. The relevant functional departments will carry out a follow-up audit when there is an abnormal consumption of all medical materials.
2. The personnel of each department should receive medical consumables scientifically, and implement the department responsibility system to prevent the backlog, waste, and loss of medical materials.
3. All kinds of medical consumables are uniformly allocated by the pharmacy department and the supply room, and those with the most recent expiration date will be used first. When a new variety enters the hospital, the purchasing department should be responsible for using or assisting in the handling of materials that have similar stocks.
4. The materials and medical consumables provided by the hospital are strictly prohibited to be used outside the hospital. If the outside consultation needs to be taken out, it can be purchased in the form of a prescription from the pharmacy outpatient pharmacy.
5. It is strictly forbidden for all departments and departments to enter the clinical use of medical consumables without approval procedures in our hospital. At the same time, it is also forbidden to introduce the purchase of medical consumables not supplied by our hospital to patients and their family members for any reason or name, and the consumables purchased by patients shall not be used in clinical diagnosis and treatment.
6. The medical consumables brought by experts from other institutes invited by the institute to carry out new projects must be submitted to the pharmacy department for review and price negotiation before they can be used. At the same time, the function, quality, and price of the medical consumables used should be introduced to the patient and family members in advance, and obtain the consent and signature of the patient or family members.
7. All medical consumables and low-value instruments shall not be sent directly to the department by the supplier. For implantable consumables, the issuance and acceptance should be strictly controlled, and the pharmacy department, operating room, and surgeon should check and test step by step. The suppliers of implantable consumables must deliver the materials to the pharmacy department in advance, and the pharmacy department arranger sends it to the designated person of the department to sign and receive, and make detailed use of records, archives, and books, so as to achieve the purpose of traceability at any time.
8. If the medical consumables are supplied to meet the clinical requirements, anyone should use them unconditionally. If there are any quality problems, they should report to the Pharmacy Department or relevant functional departments in time, and handle them according to relevant procedures.
9. Medical consumables for clinical trial and verification should be filled in the application according to the method of newly added medical consumables. After being reviewed and approved by the Pharmacy Department, a trial report should be written and trial report should be written within the specified time, treated as new medical consumables.
Through the above introduction, Urine Collector Factory hopes that you can simply refer to the content of this article in future use.
------------------
Deep-well Plate Factory Introduces The Requirements For The Use Of Cryovials
2022-9-28
The cryovial is made of medical polypropylene (PP) and is a disposable laboratory consumable specially used for storing biological samples. In the gaseous state of liquid nitrogen, it can withstand low temperatures to minus 187 ℃. The unique external rotation design avoids the possibility of cross-contamination. The tube cap is equipped with a silicone gasket to avoid liquid leakage, which ensures tightness even at cryogenic storage temperatures, ultimately ensuring the safety of the sample in the tube. Caps come with a variety of embeddable color codes for easy identification. The white marking area and clear scale on the tube body are convenient for users to mark and calibrate the capacity. The centrifugal force can reach 17000g. All cryovials are sterilized by gamma sterilization, DNase-free, RNase-free, and pyrogen-free. Below,Deep-Well Plate Factory Introduce the tips for using Freezing Tube :
1. The externally screwed cryovial is designed for freezing samples, and the screw cap of the externally screwed cap can reduce the chance of contamination when handling samples.
2. The internal rotation cryotube is designed for freezing samples in the liquid nitrogen phase, and the silicone gasket of the tube mouth enhances the sealing performance of the cryotube.
3. The texture of the cap makes it easy to rotate the cap.
4. The cap and body are produced with the same batch of PP raw materials, so the same expansion coefficient ensures that it can be sealed at any temperature.
5. The large marked area is convenient for writing.
6. The transparency of the tube body is extremely high, and it is easy to observe the sample.
7. The round bottom design is convenient for pouring liquid and reduces residue.
Through
the above introduction,Plastic
Reagent Bottle Factory hopes
that you can simply refer to the content of this article in future use.